While MSOT creates images based on intrinsic tissue optical properties, a particular benefit of the approach is the utilization of reporter probes for adding specificity in imaging disease biomarkers.

Target-specific moieties can be labeled with a variety of commercially available optical contrast agents. Through the wavelength-dependent absorption coefficients of the labeling agents, the concentration of the markers in the tissue of interest can be visualized with high resolution and quantified.

xPLORE probes for MSOT imaging

iThera Medical markets a proprietary line of probes optimized for the use with MSOT.

The initial portfolio of three xPLORE© probes consists of two targeted reagents for the use in disease models related to cancer and inflammation as well as one blood pool agent with long circulation time.

Apoptosis is an important mechanism in cellular homeostasis and imbalances in the apoptotic process are associated with various disease states. The xPLORE Apoptosis detection kit provides all reagents necessary to specifically detect apoptotic cells in vivo by Multispectral Optoacoustic Tomography (MSOT) imaging. The kit contains xPLORE Apo FL750 (a poly-caspase binding agent with maximal absorption at 750nm), xPLORE CTRL FL690 (a control agent that does not bind caspases and has an absorption maximum at 690nm) and xPLORE Injection buffer.

Macrophages play important roles in development as well as in adaptive and innate immune responses. Infiltration and accumulation of macrophages are processes observed in various disease states, including infection, injury, tumorigenesis, and cardiovascular and metabolic diseases. xPLORE MPh FL800 is a near infrared (NIR) absorbing (and fluorescing) molecule that has been identified through screening a diverse library of NIR dyes for binding specifically to macrophages and is designed to selectively detect macrophage by in vivo Multispectral Optoacoustic Tomography (MSOT) imaging. xPLORE MPh FL800 displays fast clearance from the blood circulation with retention at macrophage-rich regions, enabling short imaging sessions. xPLORE MPh FL800 has been used to characterize macrophage accumulation in lipopolysaccharide- (LPS) or carrageenan-induced inflammation as well as in orthotopic tumors.

xPLORE NanoTorch FL800 is an intravascular blood pool agent optimized for Multispectral Optoacoustic Tomography (MSOT). It consists of a nano-formulation with a diameter of ~100nm and is slightly negatively charged. xPLORE NanoTorch FL800 contains a near infrared (NIR) absorbing (and fluorescing) molecule, and is designed to clear from the bloodstream
slowly, so that increased vascular signal can be observed for several hours. xPLORE NanoTorch FL800 can be used to evaluate the extent of enhanced permeability and retention (EPR) in animal models of disease, because under normal conditions the formulation remains intravascular and will not extravasate. The formulation can also be used to map the first draining lymph nodes, by injecting a small dose intradermally.

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xPLORE Applications

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