The best of all imaging worlds.

iThera Medical’s proprietary Multispectral Optoacoustic Tomography (MSOT) technology provides in vivo identification of spectral signatures from multiple specific agents, along with excellent intrinsic tissue contrast. MSOT combines the molecular specificity of optical imaging with the penetration depth and spatiotemporal resolution of ultrasound:

  • Molecular specificity
    Identify and quantify disease-related biomarkers, revealing intrinsic absorbers and injected nearinfrared (NIR) probes
  • Depth and 3D resolution in real time
    Acquire whole-body images of small animals, with in-plane resolution of 150 μm, in vivo and in real time

    Comparative images – MSOT and other imaging modalities

    Left most:Distribution of exogenously introduced optical molecular probe in a mouse leg as resolved by Multispectral Optoacoustic Tomography, superimposed onto simultaneously acquired anatomical image

    Second from left: Corresponding anatomical views, made with ultrasound,...
    Second from right: ...x-ray computed tomography,...
    Right most: well as epi-fluorescence of sliced tissue, are shown for comparison.

    From Optics Letters 32(19), 2891-2893 (2007); Physics in Medicine and Biology 54(9), 2769–2777 (2009) 

    Single-wavelength vs. multispectral imaging

    By detecting optical absorption from hemoglobin and other intrinsic absorbers, MSOT offers rich anatomical contrast without the need for injecting contrast agents. Anatomical structures can thereby be imaged throughout the depth of the animal, using a single wavelength for the acquisition process.

    Single-wavelength MSOT images can offer rich anatomical information, while multispectral image acquisition allows the discrimination among absorbers with distinct optical absorption profiles.

    Wavelengths for image acquisition are chosen based on the intrinsic and extrinsic absorber spectra of interest. Spectral unmixing algorithms are then used to differentiate cross-sectional composite images into images representing individual absorbers. 

    • Mercep E et al.,
      Imaging of blood flow and oxygen state with a multi-segment optoacoustic ultrasound array,
      Photoacoustics 10 (2018) 48–53. DOI: 10.1016/j.pacs.2018.04.002.
    • Mercep E et al.,
      Combined Pulse-Echo Ultrasound and Multispectral Optoacoustic Tomography with a Multi-Segment Detector Array,
      IEEE Trans Med Imaging. 2017 May 18. DOI: 10.1109/TMI.2017.2706200.
    • Joseph J et al.,
      Evaluation of precision in optoacoustic tomography for preclinical imaging in living subjects,
      J Nucl Med. 2017 Jan 26. DOI: 10.2967/jnumed.116.182311.
    • Merčep E. et al.,
      Whole-body live mouse imaging by hybrid reflection-mode ultrasound and optoacoustic tomography,
      Opt. Lett. 40, 4643-4646 (2015). DOI: 10.1364/OL.40.004643.
    • Merčep E. et al.,
      Hybrid Optoacoustic Tomography and Pulse–Echo Ultrasonography Using Concave Arrays,
      IEEE Trans Ultrason Ferroelectr Freq Control. 2015 Sep;62(9):1651-61. DOI: 10.1109/TUFFC.2015.007058.
    • Mandal S. et al.,
      Extending Biological Imaging to the Fifth Dimension,
      Pulse, IEEE (Volume:6, Issue:3), 47-53. DOI: 10.1109/MPUL.2015.2409103.
    • Adrian Taruttis & Vasilis Ntziachristos,
      Advances in real-time multispectral optoacoustic imaging and its applications,
      Nature Photonics 9, 219–227 (2015). DOI: 10.1038/nphoton.2015.29
    • Tzoumas S et al.,
      Effects of multispectral excitation on the sensitivity of molecular optoacoustic imaging,
      J Biophotonics. 2014 Oct 3. DOI: 10.1002/jbio.201400056.
    • Subhamoy Mandal et al.,
      Optimal self-calibration of tomographic reconstruction parameters in whole-body small animal optoacoustic imaging,
      Photoacoustics, September 2014. DOI: 10.1016/j.pacs.2014.09.002.
    • Alexander Dima et al.,
      Multispectral optoacoustic tomography at 64, 128, and 256 channels,
      J Biomed Opt. 2014 Mar;19(3):36021. DOI: 10.1117/1.JBO.19.3.036021.
    • X. Luís Deán-Ben and Daniel Razansky,
      Portable spherical array probe for volumetric real-time optoacoustic imaging at centimeter-scale depths,
      Optics Express, Vol. 21, Issue 23, pp. 28062-28071 (2013). DOI: 10.1364/OE.21.028062.
    • Tzoumas S et al.,
      Unmixing Molecular Agents from Absorbing Tissue in Multispectral Optoacoustic Tomography,

      IEEE transactions on medical imaging 07/2013; DOI:10.1109/TMI.2013.2279994.
    • Buehler A et al.,

      Three-dimensional Optoacoustic Tomography at Video Rate,

      Optics Express, Vol. 20, Issue 20, pp. 22712-22719 (2012)
. DOI: 10.1364/OE.20.022712.
    • Razansky D et al.,

      Volumetric Real-time Multispectral Optoacoustic Tomography of Biomarkers,

Nature Protocols 6, 1121-1129 (2011).
 DOI: 10.1038/nprot.2011.351.
    • Ntziachristos V and Razansky D,

      Molecular Imaging by Means of Multispectral Optoacoustic Tomography (MSOT),
Chemical Reviews, 110(5), 2783-2794 (2010).
 DOI: 10.1021/cr9002566.
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