MSOT imaging offers high resolution, sub-organ visualization and quantification of contrast agents in real time, making it ideally suited for pharmacokinetic studies. Due to the noninvasive nature of MSOT and the user-independent animal positioning, longitudinal and comparable measurements across multiple scans are feasible.
Accumulation, distribution, metabolism and excretion can be imaged using targeted or untargeted contrast agents. Dyes with organ-selective excretion can be exploited to assess organ function.
The injection of a dye filtered through the kidneys can be used to create temporal color maps of concentration and maximum retention times in mice with nephropathy. ROI analysis of dye clearance can differentiate kinetics in the renal cortex and pelvis, allowing the observation of a delay in clearance kinetics in mice with nephropathy compared to control mice. Thus, MSOT can provide a surrogate marker for glomerular function.
Longitudinal quantification of ICG, which is subject exclusively to hepatobiliary excretion, allows for the non-invasive assessment of liver function in mice with APAP-induced liver injury versus healthy control animals. Reduced liver function results in a delayed excretion of ICG from the organ.
MSOT allows for the investigation of liver injury and hepatic regeneration.
Applications for Preclinical Research