RSOM for high-res gastro imaging.

In vivo optical imaging modalities are mostly limited to cell cultures, superficial tissues and intravital imaging since they lack either resolution or penetration depth. In contrast, optoacoustic (OA) imaging – combining optical contrast of optical and spatiotemporal resolution ultrasound imaging - has already been used to visualize hemoglobin in depths of around 3 cm in patients with Crohn’s disease. Realizing an even higher resolution, Raster-Scanning Optoacoustic Mesoscopy (RSOM) provides intrinsic optical tissue contrast down to 10-20 μm resolution at still high penetration depths of several millimeters.

Principle of high-resolution imaging using RSOM

A: Technical setup
B: Imaging principle
C: Low frequency (10-30MHz) detection of freshly excised liver tissue
D: High frequency (30-90MHz) detection
E: 3D-volume overlay. Bars: 1mm, XY-box: 8mm

In vivo RSOM imaging

In vivo RSOM imaging from healthy and inflamed mice. The images are reconstructed from single image planes of the murine colon (max depth: 3mm). Detected signals originate from hemoglobin (10-90MHz).

A: In vivo imaging from healthy mice; full projections (FP) YZ, XY und XZ
B: 3D volume
C: In vivo imaging from colitis; FP YZ, XZ und XZ
D: 3D volume. Bars: 1mm, XY-box: 8mm

  • Knieling F et al.,
    Raster-Scanning Optoacoustic Mesoscopy for Gastrointestinal Imaging at High Resolution
    Gastroenterology. 2018 Jan 5. DOI: 10.1053/j.gastro.2017.11.285.
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