Localize deeply seated tumor lesions.

Napp et al. show for the first time that multispectral optoacoustic tomography (MSOT) can be used in combination with an integrin targeting NIRF contrast agent preoperatively to localize deeply seated tumor lesions with high specificity and represents an ideal complement to the 2D fluorescence imaging in obtaining a complete overview of the distribution of the fluorescence signal before surgery.

A: Representative MSOT scans of an orthotopic PDAC bearing mouse are shown as transversal spectrally unmixed single-slice images and maximum intensity projections (MIPs). (1) Represents the approximate level of the liver, (2) the level of the primary tumor and (3) the bladder level. Yellow arrows point to the multiple disseminated nodules of the primary tumor, orange solid lines show the location of the tumor mass at the surgical scar, green solid lines show the location of kidneys and red dotted line the location of bladder.
B: The corresponding location of the planes 1-3 of Figure 3A is schematically depicted on a whole-body scan of the same tumor-bearing mouse taken with the IVIS optical imaging system.

Representative transversal cryoslices (Cryo) of a PDAC bearing mouse sacrificed 6h p.i. of cRGD-800CWTCO and the matching MSOT MIPs show almost identical distribution of the fluorescence (Fluo) and optoacoustic signals (MSOT) in the tumor pointed out by yellow arrows. Yellow dotted lines outline the kidneys (K), white lines the stomach (S) and white dotted lines the bladder (B).

  • Napp J et al.,
    Fluorescence- and multispectral optoacoustic imaging for an optimised detection of deeply located tumours in an orthotopic mouse model of pancreatic carcinoma,
    Int J Cancer. 2017 Dec 26. DOI: 10.1002/ijc.31236.
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