Tracking of labeled cells.

Tumor development is mainly driven by aberrant growth of cancer cells, but the contribution of the microenvironment to tumor progression is increasingly well understood. Being able to gain insights into the recruitment and persistence cells into tumor tissue is important for the understanding of tumor heterogeneity.

MSOT technology can be leveraged for tracking the tumor distribution of cells loaded with NIR-absorbing agents or genetic reports such as iRFP.

Biodistribution of injected cells

(a) Bioluminescence imaging in dorsal (top panel) and ventral (bottom panel) position after cell administration. Mirrors were placed on the side of the mouse to provide a lateral view.
(b) MSOT imaging of the same animal: 3D reconstruction for the whole animal showed the correlation between GNR-860 signal with bioluminescence imaging.

Comenge J et al., Multimodal cell tracking from systemic administration to tumour growth by combining gold nanorods and reporter genes, Elife. 2018 Jun 27;7. DOI: 10.7554/eLife.33140.

Imaging of gold nanorod labeled stem cells over time

Gold nanorods (GNRs) with a silica coating were prepared to minimize aggregation after cellular uptake by stem cells. Cells were injected in mice. MSOT Imaging of the cell clusters was performed 3 days after injection of 2 x 104 and 1 x 105 (a) and 2 x 105 GNR-Si35-labelled cells (b). The left column shows a single wavelength maximum intensity projection in the xy plane of the regions of interest. The high resolution of MSOT can be observed here, allowing visualization of small vessels (e.g. renal artery and renal vein). The middle column shows the same regions after multispectral processing, enabling high sensitivity detection of GNR-Si35 labelled cells. Volume views of the regions of interest are shown in the right column. Scale bars are 5 mm. (c) Monitoring of the growth of the 2 x 105 cell cluster over 15 days.

Comenge, J et al., Preventing Plasmon Coupling Between Gold Nanorods Improves the Sensitivity of Photoacoustic Detection of Labelled Stem Cells In Vivo, ACS Nano. 2016 Jul 26;10(7):7106-16. DOI: 10.1021/acsnano.6b03246.

Tracking tumor cell distribution via iRFP

Longitudinal tumor monitoring in a mouse that was injected with GNR-860 / reporter gene labelled cells. Localization of tumors 40 days after systemic injection of cells imaged by and MSOT (A). (B) Growth of the tumor on the left shoulder was monitored from day 33 to day 40 and is shown in 3D reconstruction. Color scale in (A) is 1.2 to 14 MSOT intensity units (a.u.).  

Comenge, J et al., Multimodal cell tracking from systemic administration to tumour growth by combining gold nanorods and reporter genes, Elife. 2018 Jun 27;7. pii: e33140. DOI: 10.7554/eLife.33140.

Imaging macrophage uptake into tumor

Bone marrow cells were isolated from the femur of a BALB/c nude mouse and differentiated into macrophages by tumor cell conditioned media. Cells were then labeled using the CellVue® NIR 815 cell labeling kit and injected systemically (0.5x106 cells) in a BALB/c nude mouse bearing an orthotopic 4T1 breast tumor. MSOT imaging was performed before and after injection (10 min. and 24 hrs).

A:
Macrophage accumulation in tumor was visualized after 24 hrs.
B: Determination of the key components within the MSOT data by PCA/ICA analysis confirmed that the main signal consisted of labeled cells.
C: Quantification of macrophage signal by linear regression showed a significant accumulation of cells after 24 hrs.

 

  • Comenge J et al.,
    Multimodal cell tracking from systemic administration to tumour growth by combining gold nanorods and reporter genes,
    Elife. 2018 Jun 27;7. DOI: 10.7554/eLife.33140.
    Link
  • Comenge J et al.,
    Preventing Plasmon Coupling Between Gold Nanorods Improves the Sensitivity of Photoacoustic Detection of Labelled Stem Cells In Vivo,
    ACS Nano. 2016 Jun 16. DOI: 10.1021/acsnano.6b03246.
    Link
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