Assessment of probe clearance.

MSOT has the ability to track whole-body biodistribution and pharmacokinetics of near-infrared absorbers. This way, the accumulation and clearance of nanoparticles, dyes and/or proteins can be observed over time.

The data acquisition rate of 10 frames per second allows for the visualization of fast uptake kinetics, while longer-term data acquisition and imaging at multiple time points allows the determination of differential pharmacokinetic properties.

With the ability to visualize and quantify fast kinetics and organ specificity of injected NIR-absorbing agents of interest, MSOT is poised to become an invaluable tool in the drug discovery process by enabling whole-body in vivo visualization of drug biodistribution.

Analysis of blood plasma kinetics

Images: Regular ICG and three different formulations (Table) of liposomal ICG (50 nmol) were injected systemically and the neck region was continuously imaged for 30 min. ICG signal was unmixed by linear regression and a region of interest was placed over a large vessel in the MSOT image to determine the strength of optoacoustic signal. Values were converted to ICG concentrations by determining ICG plasma levels at T = 30 min. by fluorescence spectroscopy. The experimental data was modeled by non-compartmental analysis and half-life was calculated from the fitted curves: T1/2 = ICG (3 min.); positive liposomes (6 min.); negative liposomes (9 min.) and negative PEGylated liposomes (214 min.)

  • Taruttis A et al.,

    Fast Multispectral Optoacoustic Tomography (MSOT) for Dynamic Imaging of Pharmacokinetics and Biodistribution in Multiple Organs,

    PLoS One 2012, 7(1):ee30491.

    Link
  • Application Note: Imaging Kinetics - Fast MSOT
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