Analysis of probe biodistribution.

Biodistribution and pharmacokinetic studies are an important step in the drug discovery process. It is therefore critical to discriminate the localization of an injected drug among organs of interest.

Conventional methods involve sampling blood or organs ex vivo longitudinally followed by chemical analysis. This can be both expensive and time consuming.

With the ability to measure structures as small as 150 μm in crosssections in real time, MSOT is uniquely able to quantitatively image the kinetics and distribution of injected probes with high temporal and spatial resolution throughout the entire mouse.

Measuring probe biodistribution

Images: BALB/c nu/nu mice were intravenously injected with 25 nmol of Cy-X probe after which various clearance organs were monitored for 40-50 min. by MSOT. The signal vs. time curves were determined by region of interest (ROI) analysis as implemented in the MSOT post-processing software (bottom graphs, red circles). Kidneys, spleen, liver and other organs can readily be visualized to determine biodistribution and clearance. Standard PK parameters such as Kin, Kout and T1/2 can be determined by exporting the MSOT data and performing follow-up analysis in dedicated PK modeling software tools such as WinNonlin (bottom graphs, blue lines).

  • Taruttis A et al.,
    Fast Multispectral Optoacoustic Tomography (MSOT) for Dynamic Imaging of Pharmacokinetics and Biodistribution in Multiple Organs,
    PLoS One 2012, 7(1):ee30491.
    Link
  • Application Note: Imaging Kinetics - Fast MSOT
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