Tracking of labeled cells.

Tumor development is mainly driven by aberrant growth of cancer cells, but the contribution of the microenvironment to tumor progression is increasingly well understood. Being able to gain insights into the recruitment and persistence cells into tumor tissue is important for the understanding of tumor heterogeneity.

MSOT technology can be leveraged for tracking the tumor distribution of NIR-labeled cells. It has e.g. been used to monitor the recruitment of bone marrow derived, labeled macrophages into tumors. Also, in a recent study, MSOT imaging of gold nanorod-labeled, implanted cell clusters was shown over a time period of 15 days after injection.

Imaging of gold nanorod labeled stem cells over time

Gold nanorods (GNRs) with a silica coating were prepared to minimize aggregation after cellular uptake by stem cells. Cells were injected in mice. MSOT Imaging of the cell clusters was performed 3 days after injection of 2 x 104 and 1 x 105 (a) and 2 x 105 GNR-Si35-labelled cells (b). The left column shows a single wavelength maximum intensity projection in the xy plane of the regions of interest. The high resolution of MSOT can be observed here, allowing visualization of small vessels (e.g. renal artery and renal vein). The middle column shows the same regions after multispectral processing, enabling high sensitivity detection of GNR-Si35 labelled cells. Volume views of the regions of interest are shown in the right column. Scale bars are 5 mm. (c) Monitoring of the growth of the 2 x 105 cell cluster over 15 days.

Comenge, J., Fragueiro, O., Sharkey, J., Taylor, A., Held, M., Burton, N. C., Park, B.K., Wilm, B., Murray, P. Brust, M., Levy, R., Preventing Plasmon Coupling Between Gold Nanorods Improves the Sensitivity of Photoacoustic Detection of Labelled Stem Cells In Vivo, ACS Nano. 2016 DOI: 10.1021/acsnano.6b03246.

Imaging macrophage uptake into tumor

Bone marrow cells were isolated from the femur of a BALB/c nude mouse and differentiated into macrophages by tumor cell conditioned media. Cells were then labeled using the CellVue® NIR 815 cell labeling kit and injected systemically (0.5x106 cells) in a BALB/c nude mouse bearing an orthotopic 4T1 breast tumor. MSOT imaging was performed before and after injection (10 min. and 24 hrs).

A:
Macrophage accumulation in tumor was visualized after 24 hrs.
B: Determination of the key components within the MSOT data by PCA/ICA analysis confirmed that the main signal consisted of labeled cells.
C: Quantification of macrophage signal by linear regression showed a significant accumulation of cells after 24 hrs.

 

Publications

  • Comenge J et al.,
    Preventing Plasmon Coupling Between Gold Nanorods Improves the Sensitivity of Photoacoustic Detection of Labelled Stem Cells In Vivo,
    ACS Nano. 2016 Jun 16. DOI: 10.1021/acsnano.6b03246.
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