Imaging of glioblastoma growth.

MSOT has the capability to noninvasively image the same animal repeatedly over time, enabling longitudinal imaging. It is therefore possible to track the development of a disease such as glioblastoma.

Blood oxygenation maps can show the size and shape of the growing tumor, while molecular probes can be used to elucidate disease processes such as angiogenesis and inflammatory response. In glioblastoma, hypoxia has been associated with tumor growth and also with angiogenic and radiation resistance to therapy. Detecting brain blood oxygenation can thus support the study of the role of hypoxia in glioblastoma. In addition, it could enable the appropriate selection of therapeutic treatment.

MSOT has the penetration, specificity and resolution to detect physiological features of tumor growth through intact skin and skull.

Analysis of tumor hypoxia

Top left image: The spectrally unmixed deoxy-hemoglobin pseudocolor overlay on an 800 nm single-wavelength MSOT image from an animal 16 days following implantation with U87 glioblastoma cells. Imaging performed after 10% carbon dioxide challenge.

Top right image: Ex vivo cryosection corresponding to top left image; IntegriSense750 injected into mouse before euthanasia to visualize tumor size and location.

Bottom left image: Shows a deoxy-hemoglobin MSOT image 34 days following implantation with U87 glioblastoma cells. Deoxy-hemoglobin signals visualize the necrotic core of the tumor.

Bottom right image: Ex vivo cryosection corresponding to animal in bottom left image showing tumor boundaries and the beginning stages of tumor necrosis.

Burton NC, Patel M, Morscher S, Driessen W, Claussen J, Beziere N, Jetzfellner T, Taruttis A, Razansky D, Bednar B, Ntziachristos V, Multispectral Optoacoustic Tomography (MSOT) Brain Imaging and Characterization of Glioblastoma, Neuroimage, 2012 Sep 28; pii: S1053-8119(12)00963-9.

  • Shang W et al.,
    Core-Shell Gold Nanorod@Metal-Organic Framework Nanoprobes for Multimodality Diagnosis of Glioma,
    Adv Mater. 2016 Nov 18. DOI: 10.1002/adma.201604381.
    Link
  • Gao S et al.,
    A non-viral suicide gene delivery system traversing the blood brain barrier for non-invasive glioma targeting treatment,
    J Control Release. 2016 Oct 26. DOI: 10.1016/j.jconrel.2016.10.027.
    Link
  • Burton NC et al.,
    Multispectral Optoacoustic Tomography (MSOT) Brain Imaging and Characterization of Glioblastoma,

    Neuroimage, 2013 Jan 15;65:522-8. DOI: 10.1016/j.neuroimage.2012.09.053.
    Link
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